Noncytopathic mutants of Newcastle disease virus

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Replication of Newcastle Disease Virus

Infection of L cells with Newcastle disease virus (NDV) results in abortive replication, with the production of very few plaque-forming units (PFU) of virus per cell (W. C. Wilcox, Virology 9:30, 1959; K. Paucker et al., Virology 17:301, 1962). It is known that interferon is synthesized by L cells infected with NDV, and the possibility exists that this endogenously produced inhibitor may be res...

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Efficacy of Thermostable Newcastle Disease Virus Strain I-2 in Broiler Chickens Challenged with Highly Virulent Newcastle Virus

Newcastle disease (ND) is a major threat to poultry industry production throughout developing countries. The Newcastle disease viruses (NDVs) infecting industrialized and indigenous poultry in Iran are velogenic strains and responsible for the frequent outbreaks of ND in poultry farms even in vaccinated flocks causing serious economic losses in the commercial and indigenous poultry. However, va...

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Studies on Newcastle Disease Virus

1. It is likely that certain tailed and filamentous particles seen on electron microscope examination of partially purified saline suspensions of Newcastle virus are the individual virus particles because: (a) They have a highly characteristic shape not seen in other virus preparations. (b) They are present whenever the virus is present in high concentration. (c) Their size agrees with the size...

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Virion functions of RNA+ temperature-sensitive mutants of Newcastle disease virus.

Virions from Newcastle disease virus mutants in four temperature-sensitive RNA+ groups were grown in embryonated hen eggs at the permissive temperature, purified, and then analyzed for biological properties at both the permissive and nonpermissive temperatures. At the permissive temperature, virions of mutants in groups B, C, and BC (11 mutants) were all lower in specific (per milligram of prot...

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Newcastle disease virus infection of L cells.

Newcastle disease virus (NDV) California strain reportedly grows poorly in L cells but replicates very well in chicken embryo cells. NDV-infected L cell cultures show a characteristic virus growth curve with respect to uridine incorporation, but plaque assays of the virus produced 24 h postinfection (PI) show no infectious particles when assayed on L cell monolayers and only a very low titer on...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1978

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.26.3.724-729.1978